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Researchers at Max Planck Florida Institute for Neuroscience have developed new software to study synaptic plasticity in dendritic spines
When humans and animals learn and form memories, the physical structures of their brain cells change. Specifically, small protrusions called dendritic spines, which receive signals from other neurons, can grow and change shape indefinitely in response to stimulation. Scientists at Max Planck Florida Institute for Neuroscience (MPFI) have observed this process, known as long-term structural plasticity, in individual spines, but doing so requires substantial time and effort. A new technique, developed by MPFI researchers, automates the process to make observing and quantifying this growth far more efficient. The open-source method is available to any scientist hoping to image plasticity as it happens in dendritic spines using Scanimage. The work was published in January 2017 in the Public Library of Science journal, PLOS ONE.
Scientists working in Ryohei Yasuda’s laboratory at MPFI are working to understand how proteins facilitate the plasticity of dendritic spines, the biological basis of learning and memory. They use 2-photon microscopy, an advanced technique for live-cell imaging, and glutamate uncaging, a technique that can induce plasticity in individual spines of interest using light. This is a meticulous process, wherein a scientist must continually focus the microscope on a single dendritic spine over an extended period, often an hour or longer. Michael Smirnov, Ph.D., Post-doctoral researcher at MPFI, developed a software that allows the computer to automatically track, image, and stimulate up to five dendritic spines at a time. “We can collect the data and figure out the proteins responsible much quicker with this [program] because we can run much more robust experiments,” said Smirnov. In addition to increasing productivity, the ability to stimulate and image multiple spines in parallel greatly decreases the cost of running these experiments.
In contrast to previous open-sourced focusing programs, this one implements a highly capable and customizable focus and drift correction system to ensure that it can be used for a variety of biological applications. “The paper explains further modifications to make the process automated,” said Smirnov. “It shares the open source code, so essentially, other people from other institutes can easily pick this up and use it for themselves.”
Smirnov, M.S., Evans, P.R., Garrett, T.R., Yan, L., and Yasuda, R. (2017). Automated Remote Focusing, Drift Correction, and Photostimulation to Evaluate Structural Plasticity in Dendritic Spines. PLOS ONE 12, e0170586.