We designed a head-mounted three-photon microscope for imaging deep cortical layer neuronal activity in a freely moving rat. Delivery of high-energy excitation pulses at 1,320 nm required both a hollow-core fiber whose transmission properties did not change with fiber movement and dispersion compensation. These developments enabled imaging at >1.1 mm below the cortical surface and stable imaging of layer 5 neuronal activity for >1 h in freely moving rats performing a range of behaviors.
You may also like
New Research Shows How Cultural Transmission Shapes...
March 27, 2023Max Planck Institute for Empirical Aesthetics
Mapping unknown territory
February 27, 2023Max Planck Institute for Biological Intelligence
Uridine makes you hungry
January 23, 2023Max Planck Institute for Metabolism Research
Amygdala Intercalated Cells: Gatekeepers and Conveyors...
January 19, 2023Max Planck Florida Institute for Neuroscience
Aversive bimodal associations differently impact...
January 19, 2023Max Planck Institute for Chemical Ecology
Commonalities and Asymmetries in the Neurobiological...
January 5, 2023Max Planck Institute for Psycholinguistics