Two-photon (2P) imaging has proven to be a powerful tool for investigating neural structure and function both in brain slices and in intact systems. In vivo 2P imaging presents significant challenges in sample preparation, which are exacerbated in non-murine species. Here, we describe procedures for the effective virally mediated labeling of neurons and for the implantation of cranial windows for imaging. The procedures described here are applicable to a range of species, including mice, and are routinely used in ferrets and tree shrews to provide large-scale labeling of cortical volumes and high-quality imaging data.
You may also like
Amygdala Intercalated Cells: Gatekeepers and Conveyors...
January 19, 2023Max Planck Florida Institute for Neuroscience
In the zone for memories
January 10, 2023Max Planck Institute for Brain Research
Neuroscientists illuminate how brain cells deep in the...
December 1, 2022Max Planck Institute for Neurobiology of Behavior – caesar
How visual information travels from the retina to the...
October 6, 2022Max Planck Institute for Biological Intelligence
Different flavors of inhibition save the day
August 23, 2022Max Planck Institute for Brain Research
Gamma rhythms – a phenomenon simpler than...
August 1, 2022Ernst Strüngmann Institute for Neuroscience